DOI > 10.5291/ILL-DATA.1-20-46

This proposal is publicly available since 02/25/2022

Title

Development of a method for matchout labelling of DNA for SANS stuctural studies of DNA-protein complexes

Abstract

Here we propose to study DNA extracted from a range of deuterated cultures to identify culture conditions that result in matchout labelled DNA. Such information has been developed for proteins and is now very widely used. Matchout labelled DNA would be of major importance for the study of protein/nucleic acid complexes and will further broaden the application of SANS methods for the study of biological systems.

Experimental Report

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Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

BROCCO Benjamin; Juliette M. Devos; FORSYTH Victor Trevor; HAERTLEIN Michael and MARTEL Anne. (2017). Development of a method for matchout labelling of DNA for SANS stuctural studies of DNA-protein complexes. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.1-20-46

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Metadata

Experiment Parameters

  • Environment temperature

    6 - 50 °C

  • Experiment energy

    7

Sample Parameters

  • Formula

    • DNA
    • Protein

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