Investigating tethered lipid bilayer biosensor platforms
Bruce Cornell has previously developed a bilayer system that is covalently bonded to a gold substrate and which is extremely stable and optimized for incorporating transport proteins [1]. That tethered bilayer system comprises a mixture of synthetic archaebacterial double-length reservoir half-membrane-spanning phytanyl lipids (DLP) and full-membrane-spanning lipids (MSL) which are attached to a gold surface via polar linkers and sulphur-gold bonds. The surface density of those tethered species is controlled by dilution with a low-molecular weight hydrophilic spacer, mercaptoacetic acid disulphide (MAAD) or ethylene disulphide (EDS) which are directly attached to the gold surface using the same chemistry. We have used impedance spectroscopy to characterize the functional properties of membrane transport proteins that are incorporated in that tethered bilayer system. It is important to now measure the structure of the tethered lipid bilayer system, which is the reason for applying neutron reflectivity beam time. Results will enable the properties of both the membrane and the support to be tuned for the particular requirements of specific proteins.
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MARTIN Donald; ALCARAZ Jean Pierre; MACCARINI Marco; STIDDER Barry and WATKINS Erik. (2012). Investigating tethered lipid bilayer biosensor platforms. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-632
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