Lipid organisation in the stratum corneum
The lipid matrix in the upper layer of the skin is crucial in establishment and maintenance of its barrier function. These lipid regions comprise cholesterol (CHOL), free fatty acids (FFAs) and ceramides (CERs) forming two crystalline lamellar phases with repeat distances of 5.4 nm and 12.5 nm. In previous studies performed at ILL (8-02-480, 8-02-502 and 8-02-559) we successfully resolved the scattering length density profile of the repeating unit of the 5.4 nm phase, and determined the positions within this repeating unit of the deuterated acyl chain of CER2 and two deuterated moieties of CHOL. We have also determined the scattering length density profile of the repeating unit of the 12.5 nm phase. However, as this phase is very complex, we need more information to construct a sufficiently detailed molecular model. Previously, we performed studies with CER1 having a deuterated linoleic tail. In the scheduled studies in April 2012 we will focus on the position of deuterated CHOL. To obtain a complete picture, the aim of the proposed study is to determine the position of the deuterated chain of CER2 and the deuterated fatty acids in the repeating unit in this phase.
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BARLOW David J.; BOUWSTRA Johanna Aaltje; CRISTIGLIO Viviana; DEME Bruno; g.s.gooris; M Jayne Lawrence and MOJUMDAR Enamul. (2012). Lipid organisation in the stratum corneum. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-643
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