The effect of pH on biomimetic membranes containing novel lysylphosphatidylglycerol analogues.
The bacterial lipid lysylphosphatidylglycerol (LPG) is a major component of the plasma membranes of a number of important human pathogens including Staphylococcus aureus, Mycobacterium tuberculosis and Bacillus anthracis. LPG is thought to alter the charge and order properties of the bacterial plasma membrane in order to render it resistance to a range of important cationic antimicrobials, including the defensive peptides of our own innate immune system. We are currently investigating the regulation of expression of this lipid in Staphylococcus aureus, and its influence on membrane physical properties, in particular the molecular mechanisms by which it facilitates resistance to drug partitioning. The labile nature of LPG has meant that to date, biophysical studies of its role in drug resistance have been flawed and inconclusive. At KCL we have synthesized a number of novel stable LPG analogues specifically for the purpose of modeling its role in defensive peptide resistance, using neutron reflectometry and allied techniques. In this investigation we are interested in studying the effect of environmental pH on the structure of LPG-containing bilayers and peptide resitance.
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Richard D. Harvey; BARKER Robert and REHAL Reg. (2013). The effect of pH on biomimetic membranes containing novel lysylphosphatidylglycerol analogues.. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-666
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