Role of curvature inducing lipids on the structural changes in model membranes caused by multivalent protein binding and receptor clustering
We propose a series of experiments to investigate the interplay between receptor clustering and the presence of positive spontaneous curvature lipid species on the structure of planar model membranes. These experiments are designed to elucidate the mechanism driving raft associated endocytosis as well as lead to the development of a switchable biomemetic signaling device. Using neutron reflectivity (NR) and in-situ impedance spectroscopy NR, we will investigate structural changes induced by CTxB binding and resulting receptor clustering on lipid membranes containing lyso-PC lipids. Preliminary NR experiments found that CTxB binding increased the bilayer thickness and decreased the bilayer coverage on the substrate (by ~10%) and that these two parameters changed in such a way as to conserve the overall lipid volume of the sample. This preliminary work will be extended to confirm the hypothesized membrane strcutral changes and to measure the associated transmembrane electrical responses which may be exploited for future use in biomemetic devices.
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WATKINS Erik and BARKER Robert. (2013). Role of curvature inducing lipids on the structural changes in model membranes caused by multivalent protein binding and receptor clustering. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-684
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