Magnetic contrast reflectometry to resolve transmembrane potential effects on the binding of MinD-MTS to a floating bilayer
Magnetic contrast neutron reflectometry will be used to resolve the effect of an applied transmembrane potential on a floating bilayer and its interaction with the MinD-MTS peptide which is involved in the control of bacterial cell division. The floating bilayer will be deposited on a gold layer capping a permalloy under layer on a silicon block. The gold layer will serve as the working electrode in an electrochemical cell, permitting application of transmembrane potentials in the range -100mv to -1V, which span the range normally found across the cytoplasmic membrane of bacterial cells. We aim to demonstrate that such an applied potential leads to a change in the lipid tilt and area per molecule in the bilayer, that effectively increases the fluidity (lowers the lateral pressure), providing a structural mechanism to bridge between the observations that the binding of fluorescent MinD in cells is affected by transmembrane potential and MD simulations of changes in bilayer fluidity with applied potential.
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TITMUSS Simon; BARKER Robert and MCKINLEY Laura. (2014). Magnetic contrast reflectometry to resolve transmembrane potential effects on the binding of MinD-MTS to a floating bilayer. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-704
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