DOI > 10.5291/ILL-DATA.8-02-719

This proposal is publicly available since 09/21/2020

Title

Molecular adaptation of plasmic membrane in archael piezophile

Abstract

In the past, neutron experiments proved to be successful in quantifying the extent to which macromolecular dynamics in bacterial cells is affected by adaptation to extreme temperatures and salinity (Tehei et al., 2004; Tehei et al., 2007). The results supported the hypothesis that the evolutionary selection of appropriate resilience in order to maintain macromolecular structure and flexibility within the narrow limits required by biological activity contributes to environmental adaptation. Archael piezophiles have the ability to tune the composition of their membranes in order to adapt to the external conditions (temperature and pressure). It has been shown that the composition of the membrane changes depending on the growth conditions, so that the membrane can maintain its fluidity. Preliminary data shows us that the phase transition temperatures depend greatly on the temperature and pressure at which the organism was cultivated. The objective of our project is to map these phase transitions as a function of growth conditions by monitoring the d-spacing value of these membranes as function of temperature.

Experimental Report

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Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

Phil Oger; CARIO Anais; DEME Bruno; MARTINEZ Nicolas and PETERS Judith. (2015). Molecular adaptation of plasmic membrane in archael piezophile. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-719

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Metadata

Experiment Parameters

  • Environment temperature

    10 - 85 °C
  • Experiment energy

    4.7 A

Sample Parameters

  • Formula

    • Lipids extracted from E. coli and T. barophilus