Neutron diffraction studies of membrane rafts
The lateral organisation of lipids and proteins in the eukaryotic cell membrane has been the focus of much recent research. Of particular interest is how some lipid components of the membrane can phase separate to form highly dynamic, nanoscale structures known as lipid rafts. The present study uses neutron diffraction to study the effect of temperature and composition on the formation of rafts prepared using dioleoylphosphatidylcholine (DOPC), cholesterol (chol) and dipalmitoylphosphatidylcholine (DPPC). Significantly these studies will enable the thickness of the different domains present in the raft to be determined; information that cannot be obtained using other techniques available to study rafts. These studies will complement other neutron scattering studies and enable us to build up a detailed picture of molecular architecture of rafts in bilayer membranes.
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M Jayne Lawrence; BARLOW David J.; DEME Bruno and FRAGNETO Giovanna. (2015). Neutron diffraction studies of membrane rafts. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-720
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