SANS studies of clathrin-mediated endocytosis
Clathrin-mediated endocytosis is crucial for the internalization of most eukaryotic cell-surface proteins. Clathrin-coated vesicles (CCV) assemble with their cargo at the plasma membrane then transport these to the early endosome inside the cell. CCV consist of a clathrin scaffold and a lipid vesicle containing the cargo, linked by adaptor proteins that are associated with effectors of CCV assembly, stability and disassembly. We recently determined that a single adaptor protein AP2 is sufficient to initiate and drive clathrin-coated bud formation on appropriate membranes, enriched in PtdIns(4,5)P2 (Kelly et al., Science, 2014). The resultant buds were spherical and uniform in size (approximately 400-Ångstrom radius). This technical advance has now put us in a position where we can generate clathrin-coated vesicles with known protein and lipid composition. The opportunity to study simpler and more homogeneous CCVs by SANS will allow us to determine the structural effect of factors that influence CCV formation, size and disassembly.
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ZACCAI Nathan; MARTEL Anne; OWEN David J. and Antoni G. Wrobel. (2015). SANS studies of clathrin-mediated endocytosis. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-728
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