Dependence of Amphotericin B activity in Pichia Pastoris yeast on membrane sterol content
We have recently carried out a detailed characterization of the lipid composition and structure of native fungal membrane extracts from Pichia Pastoris, as well as the structural consequences of the antifungal agent AmB action on yeast phospholipid membranes. The structure of yeast phospholipid membranes differs considerably from typical model lipid bilayers composed of synthetic lipids and depends on the degree of lipid polyunsaturation as well as the presence of the native ergosterol. AmB inserts in yeast membranes both in the absence and presence of ergosterol, and forms a thick extra-membraneous aggregate in agreement with a recently proposed new model in which it acts as an ergosterol-extracting sponge. We propose to continue the previous investigation by using total lipid extracts from P. pastoris, which also include the other non-polar lipids (diglycerides, sterol esters), and will investigate the effect of membrane sterol content on the mechanism of AmB
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WACKLIN KNECHT Hanna; DELHOM Robin and FRAGNETO Giovanna. (2015). Dependence of Amphotericin B activity in Pichia Pastoris yeast on membrane sterol content. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-746
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