Real-time monitoring of the change in lipid vesicle structure upon amyloid fibril formation
We are aiming to apply small angle neutron scattering in order to study whether or not the nucleation of alpha-synclein amyloid fibrils in presence of lipid SUVs leads to the formation of lipid-protein co-aggregates. In particular, using contrast matching of the protein and by following the time evolution of the form factor of the lipids, we focus on the question whether the lipid molecules remain quantitatively within spherical vesicles or whether, at which stage and in which manner they are partly incorporated into the growing fibrils. Co-aggregation of lipids and amyloid fibrils can lead to disruptions of the lipid membrane and could hence be at the origin of the toxicity induced by the aggregation of alpha-synclein that ultimately leads to neuronal death. The proposed experiment will thus tackle a very basic question of protein science, with potential outreach for a better understanding of neurodegenerative diseases.
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GALVAGNION Celine; Alexander K. Buell; DOBSON Christopher; MARTEL Anne and ROOSEN RUNGE Felix. (2015). Real-time monitoring of the change in lipid vesicle structure upon amyloid fibril formation. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-754
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