Quantifying Physical Communications between Model Membranes of Pluripotent Stem Cells via Surface Saccharides
During embryonic development, several kinds of saccharides, SSEA (stage-specific embryonic antigen) marker on cellular surfaces, are expressed and used for a pluripotency marker. In spite of an increasing knowledge on unique expression pattern of saccharide antigens displayed on pluripotent stem cell surfaces, no quantitative studies have been performed for pluripotent stem cells due to complex chemistry of surface saccharides and difficulties in measuring weak binding between saccharides. To understand how pluripotent stem cells communicate with the neighboring stem cells and their surrounding environments, it is essential to understand the functions of characteristic moieties expressed on their surfaces. The aim of the proposed experiment is to quantitatively understand how the SSEA moieties on phospholipid membranes control the mechanical properties of membranes. Compression rigidity and bending rigidity, which represent the vertical interaction potential between membranes and intra-membrane potential of membranes, respectively, will be obtained from scattering intensity profiles.
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YAMAMOTO Akihisa; ABUILLAN Wasim; DEME Bruno; MIELKE Salomé; SADAKANE Koichiro and TANAKA Motomu. (2016). Quantifying Physical Communications between Model Membranes of Pluripotent Stem Cells via Surface Saccharides. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-760
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