Quantification of sterol removal by amphotericin B and impact of sterol content on its interaction with natural yeast membranes
The activity of Amphotericin B against systemic fungal infections is widely accepted to result from its specific interaction with ergosterol. We have recently carried out a detailed characterization of the lipid composition and structure of native fungal membrane extracts from Pichia pastoris, as well as the consequences of AmB action. The structure of yeast membranes differs considerably from typical model lipid bilayers composed of synthetic lipids and depends on the degree of lipid polyunsaturation. AmB inserts in yeast membranes both in the absence and presence of ergosterol, and our results confirmed directly that AmB forms a thick extramembraneous aggregate, proposed recently to act as an ergosterol-extracting sponge. We propose to complete our investigation of P. pastoris membranes by quantifying the effect of the different lipid fractions (polar/apolar lipids, sterols) on the ergosterol and cholesterol extraction and AmB insertion by using membranes where the lipid fractions and sterol are selectively labeled.
Please note that you will need to login with your ILL credentials to download the data.
Download DataThe recommended format for citing this dataset in a research publication is in the following format:
DELHOM Robin; FRAGNETO Giovanna and WACKLIN KNECHT Hanna. (2016). Quantification of sterol removal by amphotericin B and impact of sterol content on its interaction with natural yeast membranes. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-773
ILL has partnered with DataCite as the registration agency
for data persistent identifiers.