Location of ubiquinone coenzyme Qn in lipid bilayers
Ubiquinones are linear polyisoprenoid derivatives of quinones that act as electron carriers in many bioenergetics processes, but also as cofactors for enzymes that are targets for a number of anti-proliferative and anti-inflammatory drugs in clinical use. It is generally believed that the branched isoprenyl units of Q10 reside in the hydrophobic centre of membranes, aligned perpendicular to the lipid chains. This raises the question how membrane-bound enzymes such as dihydroorotate dehydrogenase (DHODH), whose catalytic domain resides outside the cell membrane, access the membrane-bound ubiquinones. We are starting a new research project aiming to elucidate the structure and function of membrane-bound DHODH reconstituted into well-defined mitochondrial membrane mimics. As a pre-study we first need to determine the structure of ubiquinone-containing membranes. In this experiment our aim is establish the location of short and long-chain ubiquinones Q2 and Q10 in d82-POPC membranes and deuterated mimics of the inner mitochondrial membrane environment of DHODH.
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WACKLIN KNECHT Hanna; FRAGNETO Giovanna and KNECHT Wolfgang. (2016). Location of ubiquinone coenzyme Qn in lipid bilayers. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-775
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