Using the nano discs approach at surfaces to determine the relation between substrate specificity and the conformation of cytochromes P450 m
Nanodiscs films are useful to study the conformation of membrane bound proteins as a function of lipid membrane charge. We have studied systematically the best protocol to form dense nanodiscs films on silica by changing parameters such as the protein belt type, the lipid type, the incubation time and the temperature of incubation. We have now finally obtained a protocol that ensure more than 80% nanodiscs coverage for samples containing membrane bound proteins. This protocol will now allow us to study a series of plant membrane bound enzymes (CYPs) that are involved in several reactions in the production of important metabolites. The protocol already established will now allow to systematically study the overall conformation of the protein in the lipid bilayer (how much it is buried in the bilayer) as a function of bilayer charge, and correlate with the activity of these proteins.
Please note that you will need to login with your ILL credentials to download the data.
Download DataThe recommended format for citing this dataset in a research publication is in the following format:
CARDENAS; Richard A. Campbell; HEDEGAARD Sofie; JAGALSKI Vivien and Tania Kjellerup Lind. (2016). Using the nano discs approach at surfaces to determine the relation between substrate specificity and the conformation of cytochromes P450 m. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-776
ILL has partnered with DataCite as the registration agency
for data persistent identifiers.