Protein and DPPC competitive adsorption can be regulated by fluorocarbon gases and sinusoidal oscillations close to respiration frequencies
It is appreciated that proteins such as albumin and fibrinogen interfere with the adsorption of components of lung surfactant to the surface of the alveoli, thus disrupting normal pulmonary function, which can lead to serious consequences. Recently we have shown that direct interactions of films with biocompatible fluorocarbon gases under sinusoidal oscillations of the surface area of films appear to inhibit these effects as the surface pressure then converges on values closer to that of lipid than protein. A direct quantification of these effects is however missing to date. Here we propose to exploit new methodology developed recently on the high flux FIGARO reflectometer at the ILL to resolve the composition at the air/water interface of mixed protein/lipid systems in situ while they are subjected both to fluorocarbon gases and to sinusoidal oscillations. The strong scattering from the fluorocarbons and deuterated lipid will be exploited. This work will give important new insight into the fundamental mechanisms by which proteins inhibit lung function, and pave the way for studies on more complex systems involving lung surfactant targeted therapies in the future.
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LIU Xianhe; Richard A. Campbell; COUNIL Claire; HASSINI Rana; KRAFFT Marie-Pierre; Mendoza-Ortega Estefania E.; MIELKE Salomé; SHI Da; TANAKA Motomu and Andrea V. Vela-Gonzalez. (2016). Protein and DPPC competitive adsorption can be regulated by fluorocarbon gases and sinusoidal oscillations close to respiration frequencies. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-777
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