Membrane lipid composition and antifungal drug resistance in pathogenic yeast
The yeast Candida glabrata has became a main cause of mucosal and systemic infections during recent decade, but its virulence and resistance factors are so far only poorly understood.We have recently developed a set of RNA interference tools that allow us to study the virulence and resistance mechanisms in C. glabrata at the genetic level by upregulating/downregulating specific genes, as well as the lipidomic tools to determine the corresponding changes in membrane lipid and sterol composition. This project builds on the results obtained in our recent study using membranes from the model yeast Pichia pastoris, which showed that the AmB mechanism of interaction is strongly influenced by both the phospholipid and sterol composition of the membranes. The aim of this proposal is to investigate the role of the different lipid components of C. glabrata membranes and the structural basis of AmB activity in membranes derived from two C. glabrata strains manipulated with our RNAi tools so that they are more susceptible or resistant to AmB respectively than the wild type.
Please note that you will need to login with your ILL credentials to download the data.
Download DataThe recommended format for citing this dataset in a research publication is in the following format:
WACKLIN KNECHT Hanna; DELHOM Robin; FRAGNETO Giovanna; HALCROW William; ISHCHUK Olena; KNECHT Wolfgang; Katarina Koruza; LUCHINI Alessandra and NIGHTINGALE James. (2016). Membrane lipid composition and antifungal drug resistance in pathogenic yeast. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-787
ILL has partnered with DataCite as the registration agency
for data persistent identifiers.