Lamellar neutron diffraction studies of a simplified (4-component) model stratum corneal system
The lipid matrix in the upper layer of the skin is crucial to the skin’s barrier function. This matrix contains cholesterol (CHOL), free fatty acids (FFAs) and ceramides (CERs), arranged as lamellar phases with repeat distances of ~5.4 nm (SPP) and ~12.5 nm (LPP). In previous studies performed at ILL, we successfully resolved the scattering length density profiles of the SPP and LPP, and subsequently determined the positions within the LPP phase of the FFA, CER2 acyl chain, CHOL, and CER 1 linoleate. The LPP studied previously was prepared from a mixture of six CERs, CHOL, and FFA, and was found to have a complex structure involving three lipid layers, with water at the boundaries of the unit cell and in the interior. In recent X-ray diffraction studies, we have now established that the LPP can be prepared using only 4 components, with CER1 in combination either with CER2 or CER3. However, the repeat distance varies between 12.4 and 13.6 nm, and so it is uncertain whether the CERs and CHOL are arranged as found within the 8-component, three-layer LPP. In this proposal we will focus on the simplified 4-component mixture and determine the positions of the CER2, CER3, and CER1.
Please note that you will need to login with your ILL credentials to download the data.
Download DataThe recommended format for citing this dataset in a research publication is in the following format:
BARLOW David J.; AHMADI Delaram; BEDDOES Charlotte; BOUWSTRA Johanna Aaltje; DEME Bruno; g.s.gooris and M Jayne Lawrence. (2017). Lamellar neutron diffraction studies of a simplified (4-component) model stratum corneal system. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-788
ILL has partnered with DataCite as the registration agency
for data persistent identifiers.