Neutron fiber diffraction analysis of the structural dynamics of microtubules under physiological conditions
Microtubules are key components of the cytoskeleton in eukaryotic cells, which structure is closely related to many cell functions, e.g., migration, shape changes, differentiation and mitosis, as well as to their abnormal functioning in the case of cancer cells. Here, using a new technique for the rapid shear-flow alignment of microtubule, we would like to investigate how the configuration change of microtubule subunits (tubulin) is occurring during the assembly of microtubules, chemical reactions (GTP hydrolysis, binding to paclitaxel) and disassembly. This novel approach we try here is expected to provide us with a new tool to investigate the structural dynamics of native microtubules including the effects of tubulin-targeting anti-cancer agents.
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Kamimura S; CRISTIGLIO Viviana; DEME Bruno and PORCAR Lionel. (2017). Neutron fiber diffraction analysis of the structural dynamics of microtubules under physiological conditions. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-790
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