Deuterated cholesterol and final contrasts for completeness of the investigation of AmB mechanism within natural yeast biomembranes.
It is widely accepted that the activity of Amphotericin B (AmB) results from its specific interaction with ergosterol. We have carried out a detailed characterization of the lipid composition as well as the structural consequences of AmB over native fungal extracts from Pichia pastoris. The structure of yeast membranes differs considerably from typical model lipid bilayers composed of synthetic lipids. AmB inserts in yeast membranes both in the absence and presence of ergosterol, and our results confirmed directly that AmB forms a thick extramembranous aggregate, proposed recently to act as an ergosterol-extracting sponge, only in presence of the complex natural lipid mixture. We propose to make advantage of the new fully deuterated cholesterol available from D-Lab in order to complete our measurements and quantify the differences of AmB action between ergosterol and cholesterol-containing reconstituted biomembranes, proposed to be the origin of AmB toxic side effects in mammalian cells. We will also investigate the impact of the surrounding apolar lipids that are present in addition to the phospholipids in biological membranes, by performing two yet untried contrasts.
Please note that you will need to login with your ILL credentials to download the data.
Download DataThe recommended format for citing this dataset in a research publication is in the following format:
DELHOM Robin; FRAGNETO Giovanna and WACKLIN KNECHT Hanna. (2017). Deuterated cholesterol and final contrasts for completeness of the investigation of AmB mechanism within natural yeast biomembranes.. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-796
ILL has partnered with DataCite as the registration agency
for data persistent identifiers.