Out-of-equilibrium active membranes: the incorporation of bacteriorhodopsin in a floating lipid bilayer
Membrane proteins are associated with the lipid membranes in different ways, thus performing numerous specific functions. Using metabolic energy as photochemical reaction, transmembrane proteins frequently undergo conformational changes to perform their functions. Such protein activity leads to out-of-equilibrium fluctuation of the complex systems consisting in integrated proteins and lipid bilayers. Measurement of the fluctuation spectrum at sub-µm length scales is needed to complete understanding of the undergoing active processes. To investigate out-of-equilibrium fluctuations of phospholipid membranes induced by active transmembrane proteins, we propose to study the insertion of bacteriorhodopsin (BR) in floating phospholipids bilayers. The detergent-mediated incorporation method is in use to accomplish the most crucial step for this project – incorporate BR, a light driven proton pump, in the floating bilayer. NR will allow us to finely characterize the induced changes in the static structure of the double bilayer system caused by the protein incorporation and activity. This experiment will be a major step to achieve a better understanding of out-of-equilibrium fluctuations.
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MUKHINA Tetiana; charitat; DAILLANT Jean; FRAGNETO Giovanna and GERELLI YURI. (2018). Out-of-equilibrium active membranes: the incorporation of bacteriorhodopsin in a floating lipid bilayer. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-803
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