Flexible Modulation of Anisotropic Mechanical Properties of Membrane Stacks Separated by Hydrogels: Off-Specular Neutron Scattering Study
To physically model how cell-cell contacts establish a finite distance with aid of biopolymers, we design a well defined model based on planar bilayer stacks whose periodicity can be flexibly tuned by the mixing ratio of amphiphilic DGI and hydrophilic AAm monomers. An anisotropic hydrogel can be fabricated under shear stress, showing a highly uniform lamellar stacks of DGI whose periodicity can reach even up to hundreds of nm. The goal of the proposed experiment is to quantitatively unravel how the anisotropic mechanical parameters of membranes, such as bending rigidity and vertical compression modulus, are modulated by changing the DGI/AAm ratio in the presence and absence of external osmotic pressure. To gain specular/off-specular scattering signals from such lamellar systems possessing the periodicity of about 10 nm, we utilize D16 membrane diffractometer equipped with a high-precision humidity control system.
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YAMAMOTO Akihisa; ABUILLAN Wasim; DEME Bruno; KIMMLE Esther; MIELKE Salomé; SAKAMAKI Yusuke; SCHEIDERER Lukas; TANAKA Motomu and THOMA Judith. (2018). Flexible Modulation of Anisotropic Mechanical Properties of Membrane Stacks Separated by Hydrogels: Off-Specular Neutron Scattering Study. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-807
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