Constructing a comprehensive model of the archaeal membrane
To explain the stability of bilayer membranes at temperature >110°C, we proposed a membrane ultrastructure in which apolar lipids insert in the bilayer, in order to increase of membrane rigidity and decrease membrane permeability. The results of ILL experiment 8-02-762 allow us to affirm that apolar lipids are located in the inner plane of the bilayer membrane formed by the synthetic archaeal lipids DPhPC and DPhPE and the apolar isoprenoid lipid squalane. The insertion of squalane inside the membrane bears resemblance to that of glycerol in eukaryal membranes, raising questions about the influence of the proportion of apolar lipids on the behavior and ultrastructure of the archaeal membrane. This project aims investigating the dependence on the apolar lipid concentration and hydration the self-organization of archaeal membranes. Squalane will thus be incorporated at a relative concentration between 0 and 50% into membranes reconstructed from a mixture of synthetic or natural archaeal polar lipids. Membranes will be studied under varied hydration and temperature to determine membranes parameters under conditions reproducing the physiological conditions of the archaeal cells.
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SALVADOR CASTELL Marta; DEME Bruno; MISURACA Loreto; Phil Oger; PETERS Judith; TOURTE Maxime and ZELLER Dominik. (2018). Constructing a comprehensive model of the archaeal membrane. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-809
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