Neutron diffraction study of natural lipid-sterol membranes
We have recently carried out a detailed characterization of the lipid composition of native fungal membrane extracts from Pichia Pastoris [1]. We subsequently characterized the structure of membranes composed of extracts with or without ergosterol and the structural consequences induced by the antifungal agent AmB using Neutron Reflectometry measurements [2]. Notably, the structure of yeast phospholipid membranes differs considerably from those found in typical model lipid bilayers composed of synthetic lipids and their structure depends on the degree of lipid polyunsaturation as well as the presence of the native ergosterol (10-15 mol% at natural abundance). We propose to complement this study by means of Neutron Diffraction measurements as it can shed light on the organization of the lipids in single-phase or multiple-phases system as function of the external humidity. The final goal of this study, on natural lipids multilayers in which the content of cholesterol/ergosterol will be systematically varied, is the determination of the phase diagram of the mixture of natural phospholipids and ergosterol/cholesterol.
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DELHOM Robin; CRISTIGLIO Viviana; FRAGNETO Giovanna; LUCHINI Alessandra; MORRISON Rachel and WACKLIN KNECHT Hanna. (2018). Neutron diffraction study of natural lipid-sterol membranes. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-811
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