Constructing a comprehensive model of the archaeal membrane
We have recently proposed a novel membrane architecture model to explain the stability of lipid bilayers of archaeal cells at high temperatures (> 70°C) and high pressures (400 bar). In this architecture, the increase in membrane stability/rigidity is due to the presence, in the midplane of the bilayer, of apolar hydrocarbons, referred to as "apolar lipids". Our previous experiment (see ILL report 8-02-762) has demonstrated that the apolar lipid squalane can enter the membrane and is located in the midplane of the bilayer. We have started to characterize the impact of the presence of apolar lipids inside the membrane on the physicochemical properties of the bilayer using different approaches (FTIR, SAXS, DSC). We demonstrate the predicted increase in stability/rigidity, but also a concentration dependence of these variations with the apolar/polar lipid ratio. Up to now, no diffraction study has been done to determine the pressure effect on the model. Here, we would like to examine how the DoPhPC : DoPhPE lipid bilayer is affected by pressure and temperature and the physical effect of squalane under these conditions.
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SALVADOR CASTELL Marta; CRISTIGLIO Viviana; DEME Bruno; MISURACA Loreto; Phil Oger; PETERS Judith and TOURTE Maxime. (2018). Constructing a comprehensive model of the archaeal membrane. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-818
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