Probing the structure of natural phospholipid multilayers with Neutron Diffraction
Natural lipid bilayers extracted from P. Pastoris yeast cells are a relevant model system of complex biological membranes and a potential source of deuterated lipids for applications in neutron scattering techniques as well as NMR or IR-spectroscopy. During previous experiment on D16 we have characterized the total lipid extract (hydrogenous and deuterated, containing several phospholipid species, sterols, free fatty acids and triglycerides) and the phospholipid fraction (named Polar fraction). In particular during our last experiment we tested a lipid deposition method based on aqueous solution. This method strongly improved multilayer order and thus we are here proposing to complete our previous study by performing ND measurement with multilayers hydrated with different isotopic vapor compositions. This new data will allow us to extract the scattering length density profile from data analysis. In addition, recently we have achieved extraction and separation of hydrogenous and deuterated PC lipids, so we also propose to compare the Pol-multilayers with PC-multilayers in order to characterize the impact of the lipid headgroups on the multilayer structure.
Please note that you will need to login with your ILL credentials to download the data.
Download DataThe recommended format for citing this dataset in a research publication is in the following format:
LUCHINI Alessandra; BATCHU Krishna chaithanya; CRISTIGLIO Viviana; DELHOM Robin; FRAGNETO Giovanna and WACKLIN KNECHT Hanna. (2019). Probing the structure of natural phospholipid multilayers with Neutron Diffraction. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-844
ILL has partnered with DataCite as the registration agency
for data persistent identifiers.