Phospholipid bilayer of pulmonary surfactant: the effect of SP-B protein and Polymyxin B
Pulmonary surfactant is a unique mixture of phospholipids and surfactant specific proteins (< 10 %) which decreases surface tension at the alveolar air-liquid interphase. The hydrophobic protein SP-B acts as a promoter for approaching and binding of the two membrane surfaces together and stimulates formation of lamellar structures in water hypophase that are essential to facilitate the formation of surface active films. Polymyxin B (PxB), peptid based antibiotic, was found to mimic functional properties of SP-B. Oriented bilayers mimic well the biological system of interest. We propose neutron diffraction experiment to determine structural parameters of the surfactant organized in bilayers and to compare the effect of SP-B and PxB. Aligned bilayers will be prepared either from therapeutically used porcine lung surfactant (Cur) or synthetic surfactant (PPS) composed of DPPC/POPC/PLPC/DOPG. Biological experiments confirmed PPS mixture as promising substitution of an expensive porcine surfactant Cur. The aim of the experiment is to answer questions: Is there a structural similarity in Cur and PPS+SP-B? How is revealed the fusogenic effect of SP-2 and PxB? Can PxB substitute SP-B?
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UHRIKOVA Daniela; DEME Bruno; ERMAKOVA Elena; Nina Kanjaková; KUCERKA Norbert and ZELINSKA Katarina. (2019). Phospholipid bilayer of pulmonary surfactant: the effect of SP-B protein and Polymyxin B. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-847
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