Neutron reflectometry of clathrin-mediated endocytosis
Clathrin-mediated endocytosis is crucial for the internalisation of most eukaryotic cell surface proteins. Clathrin-coated vesicles (CCVs) assemble with their cargo at the plasma membrane then transport these to the early endosome inside the cell. A CCV consists of a clathrin scaffold coating a lipid vesicle, in which the cargo is bound, linked by adaptor proteins, like AP2, that are associated with effectors of CCV assembly, stability and disassembly. In vivo, AP2 solely interacts with one leaflet of the cellular membrane. Therefore, we have been able to analyse the first stages of CCV assembly by using cargo embedded in a lipid monolayer and Langmuir trough methods. This continuation proposal will now focus the NR studies onto the structure/function relationship of CCV modulators (like EPS15, FCHO2, NECAP and ARF6), which directly interact with AP2 at the cellular membrane.
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ZACCAI Nathan; CICUTA Pietro; Armando Maestro; OWEN David J. and SANTAMARIA Andreas. (2020). Neutron reflectometry of clathrin-mediated endocytosis. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-851
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