Novel high-pressure/high-temperature phases in archaeal membranes
We have recently proposed a novel membrane architecture model to explain the stability of lipid bilayers of archaeal cells at high temperatures (>70°C) and high pressures (400 bar). In this architecture, the increase in membrane stability is due to the presence, in the midplane of the bilayer, of apolar hydrocarbons. Our previous experiments (ILL report 8-02-762 and 8-02-809) have demonstrated that the apolar molecule are located in the midplane of the lipid bilayer. Using different approaches (FTIR,SAXS,DSC), we have demonstrated the impact of the presence of squalane inside the membrane on the physicochemical properties of the bilayer, and especially the predicted increase in stability/rigidity. We observed a dependence of these variations with the squalane/polar lipid ratio. Noticeably, we observe novel and yet uncharacterized structuration of the lamellar phases. We have recently demonstrated the possibility to analyze these membranes under controlled and combined pressure and temperature. Here, we would like to examine how the concentration of squalane affects the behavior of the DoPhPC:DoPhPE lipid bilayer as a function of P and T , with an ememphasis on these novel phases.
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SALVADOR CASTELL Marta; DEME Bruno; MISURACA Loreto; Phil Oger and PETERS Judith. (2019). Novel high-pressure/high-temperature phases in archaeal membranes. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-852
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