Structural investigation of natural deuterated phospholipid multilayers
Selective deuteration is a useful strategy to highlight specific sample components in different techniques such as NMR, infrared spectroscopy and neutron scattering techniques. Recently, we optimized a protocol for purifying the different phospholipid classes from the deuterated lipids extracted from yeast cells grown in deuterated culture media. In particular phosphatidycholine (dPC), phosphoethanolamine (dPE), phosphatidylserine (dPS) and Cardiolipin (dCL) lipids were successfully isolated in high yields. During our last experiment on D16, we prepared a lipid multilayer with the extracted dPC lipids and the collected experimental data showed the formation of a highly ordered multilayer. Here we propose to continue the characterization of the purified phospholipids and compare it with the results we previously reported for the total lipid and phospholipid extracts.
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LUCHINI Alessandra; BATCHU Krishna chaithanya; CRISTIGLIO Viviana and FRAGNETO Giovanna. (2020). Structural investigation of natural deuterated phospholipid multilayers. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-866
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