Kinetics of myelin membrane fusion triggered by myelin basic protein
The myelin sheath is a membrane resposible for rapid transport of signals in neurons. In multiple sclerosis, this membrane is damaged which results in neuron conduction failure. Myelin Basic Protein (MBP) which is a major component of the myelin sheath has been shown to play a major role in stabilising the myelin sheath by attaching the cytoplasmic leaflets of the myelin sheath to each other. Furthermore, a different lipid composition has been found for native and diseased membranes. In this neutron reflectivity experiment, we intend to build first a supported bilayer mimicking either cytoplasmic native or diseased myelin sheath lipid composition. We then plan to add MBP to these membranes, which then forms a layer on top of the membrane. Now we add liposomes to the system. The liposomes then fuse with the bilayer to form another membrane layer. We will perform steady state and time-resolved neutron reflectometry experiments. The kinetics of this process is characterised by a TOF neutron reflectivity measurement at different protein concentrations. By analysing the time constant of the fusion process we can obtain informations of the underlying biophysical processes.
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KRUGMANN Benjamin; GUTFREUND Philipp; HARIS Luman; Alexandros Koutsioubas; MICCIULLA Samantha; RADULESCU Aurel and Andreas M. Stadler. (2020). Kinetics of myelin membrane fusion triggered by myelin basic protein. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-870
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