Interaction of RNA with titratable bilayers
Lipid nanoparticles (LNPs) are a growing area of interest as drug delivery vehicles in mRNA therapies. The interactions of these lipids with the cargo drug delivery. Cationic ionisable lipids (CILs) are gaining interest for use in LNPs, as their interactions with their RNA cargo and with anionic lipids in endosomal membranes are theorised to play an important role in LNP formation and endosomal release respectively. Therapeutically relevant LNPs are complex, multicomponent systems with strongly pH dependent structure and interactions. We propose to use a simple system to mimic the RNA interaction with biomembranes with the use of neutron reflectometry to study the interaction. This consists of lipid bilayer containing DOTAP (cationic lipid) or MC3 (CIL) (titratable cationic lipid) and DOPC (phospholipid), which will allow us to reveal the effect of pH and charge density on the adsorption of mRNA.
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Jennifer Gilbert; Reflectometry of titratable bilayers; FRAGNETO Giovanna; Ben Humphreys; MICCIULLA Samantha; NYLANDER Tommy and SWENSON Jan. (2021). Interaction of RNA with titratable bilayers. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-892
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