A neutron reflectometry study of the interaction of glycerophospholipids and the mitochondria-integral phospholipase iPLA2-gamma
We propose to studey by neutron reflectometry the interaction of bilayers from natural extracts at the solid-liquid interface with the enzyme iPLA2-γ. The rate of PLA catalysis depends on a number of factors that includes the physical environment in which the substrate is presented as well as on the substrate's physical properties (extent of hydrophobicity and unsaturation). Since, bilayers formed from a wide range of partially deuterated molecules (varying from one another in terms of their physical properties) will be presented to the enzyme, we believe that the rate of catalysis could vary allowing us to better understand the principles dictating such preferences. Moreover, information on the substrate preference of the enzyme can be revealed, as usage of such partially (sn-1) chain-deuterated phospholipids allows us to track the lyso-lipid formation as an when they leave the bilayer upon substrate hydrolysis.
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FRAGNETO Giovanna; BATCHU Krishna chaithanya; CORUCCI Giacomo; JACOBS Robert and REED Joshua. (2020). A neutron reflectometry study of the interaction of glycerophospholipids and the mitochondria-integral phospholipase iPLA2-gamma. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-896
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