Probing CER EOS arrangement in the long lamellae of skin lipids
The lipid matrix in the skin's outer layer - the stratum corneum (SC) forms an effective barrier and comprised of ceramides (CERs), cholesterol and free fatty acids. These lipids form crystalline lamellar phases with repeating pattern of approx. 6 and 12 nm and referred to as short and long periodicity phases - SPP and LPP, respectively. In previous studies performed at ILL D16, we successfully probed molecular localization of various lipids in SPP with sufficient H2O/D2O contrast. In subsequent studies on the LPP system, the sufficient contrast allowed us to further determine molecular location of CER EOS linoleate tail in the LPP. However, due to limitation of deuteration, a full characterization of CER EOS was not possible. As we now have possibilities to have CER EOS deuterated in different regions of the molecule, we embark upon a journey with the aim of resolving CER EOS arrangement in the LPP unit cell, by taking model membrane approach and with CERs extracted from the native skin.
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The recommended format for citing this dataset in a research publication is in the following format:
NILSSON Emelie; BJORKLUND Sebastian; DEME Bruno; ENGBLOM Johan; Christopher J. Garvey and MOJUMDAR Enamul. (2021). Probing CER EOS arrangement in the long lamellae of skin lipids. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-899
This data is not yet public
This data is not yet public
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