Phospholipid bilayer of pulmonary surfactant: the effect of lipopolysaccharide and Polymyxin B
After inhalation, bacterial lipopolysaccharide (LPS) molecules interfere with a pulmonary surfactant (PS), a unique mixture of phospholipids and specific proteins (< 10 %) that decreases surface tension at the air-liquid interphase of lungs alveoli. LPS incorporated into clinically used exogenous porcine surfactant prevents the PS to reach the necessary low tension during area compression and disturbs its lamellar structures in water hypophase by swelling as resulted from our SAXS experiments. Polymyxin B (PxB), peptid based antibiotic that mimics functional properties of pulmonary specific protein SP-B, acts as an inhibitor of these structural changes. Oriented lipid bilayers hydrated from vapour mimic well the biological system of interest. We propose neutron diffraction experiment at D16 beamline to determine structural parameters of synthetic surfactant (PPS) composed of DPPC/POPC/PLPC/POPG and LPS, interacting with PxB and SP-B protein. The aim of the experiment is to specify: a) the effect of bacterial LPS on the lipid bilayer thickness of PPS and its hydration; b) the effect of PxB and SP-2 protein on PPS under pathological conditions induced by LPS.
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UHRIKOVA Daniela; DEME Bruno; Nina Kanjaková; KLACSOVA Maria; KUCERKA Norbert and ZELINSKA Katarina. (2021). Phospholipid bilayer of pulmonary surfactant: the effect of lipopolysaccharide and Polymyxin B. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-927
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