Utilizing neutron reflectivity to understand the biological function of the intrinsically disordered N-terminus of Magnesium transporter A
Knowing your enemy is essential in war, and this also applies in the war against bacterial infections. In order to develop new antibiotics, it is necessary to not only understand the antibacterial agents, but also the bacteria it is supposed to work against. Magnesium transporter A (MgtA) is a protein found in the cell membrane of S. typhimurium and E. coli. Recent studies have discovered that the N-terminus of MgtA (amino acid 1-33, from hereon called KEIF) is intrinsically disordered, but the benefit of this unstructured part is not yet clear. Thus, the aim of this study is to figure out how the intrinsic disorder of Keif contributes to the biological function of MgtA. Bulk studies of Keif have been performed using two simulation techniques (MC and MD), in combination with CD and SAXS experiments. Investigation of surface interactions have also been done using QCM-D. To continue studying the membrane interactions of Keif we would like to expand our research to include neutron reflectivity (NR). By combining all these different methods, we hope to gain a holistic understanding of the biological function of KEIF in MgtA, which in the future might lead to new effective antibiotics.
The data is currently only available to download if you are a member of the proposal team.
The recommended format for citing this dataset in a research publication is in the following format:
Marie Skepö; ERIKSSON SKOG Amanda; FRAGNETO Giovanna; GERELLI YURI; MANSSON Linda and MICCIULLA Samantha. (2021). Utilizing neutron reflectivity to understand the biological function of the intrinsically disordered N-terminus of Magnesium transporter A. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-935
This data is not yet public
This data is not yet public
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