Investigation of interaction between S2 subunit of SARS-CoV-2 Spike protein with model membranes composed of natural lipids
COVID-19 is still a poorly understood infectious disease, caused by the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Cell entry is mediated by the viral Spike Protein, which is composed of two subunits: S1 and S2. Our previous NR research focused on peptides derived from S2 that were responsible for fusion of the viral and human cell membrane during infection. However, since the Spike protein is a trimer, these experiments may not have captured any cooperative process due to the concurrent involvement of multiple S2 fusion peptides. We will therefore employ NR to investigate the membrane binding activity of the trimeric Fusion Core of the Spike S2 domain, upon its interaction with natural lipid monolayers and bilayers. The resultant data will be used to build molecular models of the Spike Protein S2 subunit bound to a planar membrane, and will therefore help clarify how lipid composition and environmental factors (such as pH) modulate Spike protein function.
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The recommended format for citing this dataset in a research publication is in the following format:
ZACCAI Nathan; BATCHU Krishna chaithanya; CORUCCI Giacomo; FRAGNETO Giovanna; Armando Maestro; MICCIULLA Samantha; ROMANO Brigida and SANTAMARIA Andreas. (2021). Investigation of interaction between S2 subunit of SARS-CoV-2 Spike protein with model membranes composed of natural lipids. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-943
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This data is not yet public
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