Salinity adaptation in halophilic archaeal membranes
Extreme halophiles can grow in conditions where the activity of water is close to 0. Despite an increased intracelullar osmolarity, a strong gradient exists between the environment and the cell, and the physico-chemical tolerance of the plasmic membrane to this osmotic stress remains unknown. Part of the increased membrane stability has been associated to the presence of two kinds of unusual archaeal lipids which have extended hydrophobic cores. These lipids could form a zipped bilayer membrane in which lipids from both faces would interact inside the midplane, with increased membrane parameter values. In this experiment, we will study reconstructed archaeal membranes to determine the impact of the proportion of extended archaeol on membrane structure and membrane resistance to high temperatures. Using these lipids, we will be able to evaluate the relative contribution of the extended lipids in membrane physical parameters in the archaeal bilayer by comparison with the 'classic' archaeal bilayer, which has already been extensively characterized.
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Phil Oger; DALIGAULT Camille; DEME Bruno; LORICCO Josephine; PETERS Judith and TOURTE Maxime. (2021). Salinity adaptation in halophilic archaeal membranes. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-952
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