Role of molecular players in protein trafficking revealed by neutron scattering
Differently-phosphorylated phosphoinositides are distinctly distributed within the eukaryotic cell. For example, PI(4,5)P2 is predominantly enriched at the plasma membrane inner leaflet, while PI(4)P is located along Golgi-endosomal trafficking axis. Acting as docking lipids, these phosphoinositides are directly involved in intra-cellular trafficking. PI(4,5)P2 binding Adaptor Proteins (such as epsin and AP2) control Clathrin-Mediated Endocytosis, which is the main mechanism by which eukaryotic cells internalize and recycle membrane proteins. Interacting with Golgi-enriched PI(4)P lipids, the oncogenic protein GOLPH-3 is essential for retrograde trafficking, including the correct localization of glycosyltransferases within the Golgi (where most glycosylation occurs in eukaryotic cells). This proposal therefore focuses on the molecular interactions between phosphoinositide-enriched lipid bilayers and AP2, epsin and GOLPH-3. In conjunction with available crystal structures, the resultant NR data will clarify the lipid-bound proteins' orientation and position, and thus provide crucial insights into their biological function.
The data is currently only available to download if you are a member of the proposal team.
The recommended format for citing this dataset in a research publication is in the following format:
SANTAMARIA Andreas; GUTFREUND Philipp; KAUFMAN Jonathan; Armando Maestro; MARIN MONTESINOS Ildefonso; OWEN David J. and ZACCAI Nathan. (2021). Role of molecular players in protein trafficking revealed by neutron scattering. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-971
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