Investigating the Kinetics of SARS-CoV-2 Membrane Fusion via Stopped-Flow SANS
The corona virus SARS-CoV-2 and the resulting COVID-19 disease have caused serious harm worldwide, in terms of both public health and socio-economic impacts. Despite the ongoing vaccination campaign, the appearance and rapid spread of new variants show that, without an effective treatment, the virus will remain a significant threat. It is therefore important to investigate and understand the mechanisms that make this virus so infectious - the fast and effective transmission between different hosts. After entering a new host, the first stage of a viral infection begins with the penetration of host cells, a process mediated by membrane fusion. We therefore propose a stopped-flow SANS experiment with contrast matched, reconstituted viral membrane vesicles and reconstituted human respiratory cell vesicles to observe the fusion kinetics. The experiment will provide us with both the timescale of the membrane fusion, and will also demonstrate under which conditions membrane fusion is either promoted or suppressed. The results will be important for the development of antiviral agents and pre-exposition prophylaxes.
The data is currently only available to download if you are a member of the proposal team.
The recommended format for citing this dataset in a research publication is in the following format:
JAKSCH Sebastian; DUBEY Purushottam Shashikumar; Henrich Frielinghaus; HOLDERER Olaf and PREVOST Sylvain. (2023). Investigating the Kinetics of SARS-CoV-2 Membrane Fusion via Stopped-Flow SANS. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-989
This data is not yet public
This data is not yet public
ILL has partnered with DataCite as the registration agency
for data persistent identifiers.