Phospholipid bilayer of pulmonary surfactant: the effect of lipopolysaccharide and Polymyxin B - II.
Bacterial lipopolysaccharide (LPS) can interfere with a pulmonary surfactant (PS), a mixture of phospholipids and specific proteins that decreases surface tension at the air¿liquid interface of lung¿s alveoli. LPS disturbs the function and structure of PS. Polymyxin B (PxB), a peptide based antibiotic, acts as an inhibitor of these changes. Oriented lipid bilayers deposited on silicon wafers and hydrated from vapour mimic well the PS lamellar structures. We performed a SAND experiment at D16 (Exp 802927) to examine structural changes of the PS model system infected by LPS and healed by PxB. Samples were hydrated by 8 % D2O showing the scattering from membranes only (water molecules being invisible). Reconstructed NSLD profiles of the bilayers indicate difference in PxB binding at lipid-water interface of PS+LPS depending on LPS content. We propose further study of the system hydrated with D2O/H2O mixtures varying the contrast between the lipid bilayers and water. The lipid bilayer thickness, the water distribution profile and the width of the bilayer ¿ water interface obtained from reconstructed NSLD profiles will elucidate the PxB binding. Experiments are related to in vivo study.
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UHRIKOVA Daniela; DEME Bruno; HUBCIK Lukas; Nina Kanjaková; KUCERKA Norbert and ZELINSKA Katarina. (2023). Phospholipid bilayer of pulmonary surfactant: the effect of lipopolysaccharide and Polymyxin B - II.. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-994
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