Structural studies of integral membrane proteins in Salipro stealth carriers
Reconstitution of integral membrane proteins (IMPs) in nanodiscs or Salipro (saposin-lipid-protein) complexes allows low-resolution structural investigation of membrane proteins in a native-like environment. In order to fully contrast-match-out the nanodisc carriers, we have recently incorporated our IMP targets in stealth carriers consisting of deuterated MSP1 belt protein and deuterated lipids. This allowed the structural characterization of our IMPs of interest in a lipid environment without the often-dominating scattering contribution of the nanodisc (Josts et al, 2018, Structure)(Nitsche et al, 2018, Communications Biology). We now further plan to adapt the stealth carrier system to the Salipro system by selectively deuterating saposin A and thus being able to contrast match out the scaffold proteins/lipids also in the Salipro system. These SANS data shall then be combined with cryo-EM data on the identical samples.
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The recommended format for citing this dataset in a research publication is in the following format:
TIDOW Henning; FORSYTH Victor Trevor; HAERTLEIN Michael; KEHLENBECK Dominique Maurice; MARIC Selma; PREVOST Sylvain and TRAORE Daouda. (2020). Structural studies of integral membrane proteins in Salipro stealth carriers. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-1010
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This data is not yet public
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