DOI > 10.5291/ILL-DATA.8-03-1058

This proposal is publicly available since 09/21/2026

Title

The role of plant prion-like domains in temperature sensing and response through liquid-liquid phase separation

Abstract

The increased average temperatures and prolonged periods of extreme heat due to global warming have already altered plant phenology for both wild and domesticated species, presenting a critical challenge for food security in the coming decades. Plants are able to perceive temperature and subsequently reprogram their growth and development for optimal reproduction and survival. However, the underlying mechanisms plants use to sense changes temperature remain poorly understood. Recent studies suggest that one important mechanism allowing a fast response to temperature stimuli is the formation of dynamic liquid-liquid phase separated states. liquid-liquid phase separation (LLPS) has become recognised as an important player with many biological functions. Intrinsically disordered proteins (IDPs) often act as drivers of phase separation in vitro and in vivo. Phase separation of IDPs depends on factors such as protein concentration, protein binding partners, ionic strength of the solution, pH and temperature. However, many studies of phase separation are merely descriptive of the process. We propose to use SANS to structurally probie IDPs in the soluble monomeric or condensed phase.

Experimental Report

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Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

MARK D TULLY; Stephanie Hutin; MARTEL Anne; MOUSSAOUI Dihia; PREVOST Sylvain and Chloe Zubieta. (2021). The role of plant prion-like domains in temperature sensing and response through liquid-liquid phase separation. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-1058

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Metadata

Experiment Parameters

  • Environment temperature

    5 to 35 degC
  • Experiment res energy

    0.004 to 0.44 Å-1

Sample Parameters

  • Formula

    • EARLY FLOWERING 3 (ELF3)