Structure of peptide-RNA condensates under passive and active conditions
Living cells are organized into distinct sub-compartments to facilitate spatiotemporal regulation of biological reactions. While this organization is traditionally thought to rely on membrane-delimited organelles, such as the nucleus and mitochondria, it also involves membraneless assemblies of proteins and nucleic acids that are kept together only by weak, dynamical interactions between their components. In the last years, groundbreaking studies suggested that these cellular assemblies have liquid-like physical properties and are formed via liquid-liquid phase separation (LLPS) processes. The objective of this project is to explore the structure of peptide-RNA condensates under varying conditions, namely at various charge ratios, temperatures and D2O contents in order to probe either peptides or RNA. We aim first at elucidating the correlation and possibly clustering peaks within condensates at the nanometered scale as well as the condensate structure at the large scale. Next, we will test an original fluidic setup that will dissociate the condensates through enzymatic reactions maintained in a steady state, thus mimicking the active milieu of the cytoplasm.
The data is currently only available to download if you are a member of the proposal team.
The recommended format for citing this dataset in a research publication is in the following format:
TRESSET Guillaume; KRA Kalouna; MARIOT Sandrine; MARTEL Anne; PORCAR Lionel and SUNDARARAJAN Tamizhmalar. (2023). Structure of peptide-RNA condensates under passive and active conditions. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-1078
This data is not yet public
This data is not yet public
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