Stealth Nanodiscs: Development of contrast optimized carrier systems for membrane proteins
In the last few years it has been demonstrated that membrane proteins can be successfully reconstituted into small membrane-like devices termed Nanodiscs. Consisting of a 10-14 nm sized phospholipid bilayer stabilized by an amphipatic protein belt, MSP, the Nanodisc stabilizes the membrane protein by providing a native-like environment. Considerable insight into the structural organization of the Nanodisc has been obtained by our group in previous experiments at ILL and ESRF through a combined SANS/SAXS approach. These data have enabled us to start optimizing the Nanodisc-carrier to be used as a platform for structural studies of membrane proteins in solution. The latest development of the system is the construction of a selectively deuterated and contrast minimized Nanodisc carrier, done in close collaboration with the D-LAB, Grenoble. This “stealth nanodisc”-carrier will then, relatively, enhance the neutron signal from the incorporated membrane protein, which is the system in focus. The aim of this proposed experiment is to establish the proof-of-concept of this “stealth Nanodiscs” approach to investigate membrane proteins.
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ARLETH Lise; FODERA Vito; FORSYTH Victor Trevor; HAERTLEIN Michael; HUDA Pie; MARIC Selma; MARTEL Anne; MERTENS Haydyn; MIDTGAARD Soren; MOLLER Magda; MOULIN Martine; NIELSEN Soeren Skou; PEDERSEN Martin Cramer and SKAR-GISLINGE Nicholas. (2013). Stealth Nanodiscs: Development of contrast optimized carrier systems for membrane proteins. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-771
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