Interaction between scFv antibodies and a biocompatible synthetic polymer
We wish to investigate the interaction between a recombinant antibody of human origin (scFv) and a synthetic polymer in free solution using SANS. Both normal and deuterated scFv will be employed. The polymer, polyvinyl pyrrolidone (PVP) is a standard antigen employed in animal studies. The aim of the experiment is to quantify previous observations, by antibody phage display and by DLS, of binding in this system. Measurements will be made of the radius of gyration and molecular weight of the PVP solutions without and with the scFv, both in the deuterated and in the natural form, at different concentrations. Since scFv tends to form large clusters in solution, use of its deuterated form, which is nearly matched by the D2O, also appreciably simplifies the data analysis.
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NIZAK Clement; DEWHURST Charles; FORSYTH Victor Trevor; GEISSLER Erik; GRILLO Isabelle; HAERTLEIN Michael; HALPERIN Avi; LASZLO KRISZTINA and SCARAMOZZINO Natale. (2013). Interaction between scFv antibodies and a biocompatible synthetic polymer. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-773
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