DOI > 10.5291/ILL-DATA.8-03-784

This proposal is publicly available since 03/15/2018

Title

The effect of confinement on proteins inside silica tubes investigated using SANS

Abstract

Confinement of bio-molecules within porous matrices is of significant interest from the biophysical point of view. These porous matrices mimic the conditions of a living cell and hence it helps in studying the various bio-physical processes taking place inside the cell. The interplay of various parameters such as protein-protein, protein-host and confined solvents significantly influence the protein conformations within the hosts. The changes in structure and conformation of protein can only be vividly accounted using neutron scattering techniques. We would like to study using small angle neutron scattering (SANS) (viz. quaternary) structure of hemoglobin entrapped in silica tubes of varying pore diameters.

Experimental Report

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Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

BHATTACHARYYA Aninda Jiban; CRISTIGLIO Viviana; GHOSH Rikhia; LINDNER Peter and MANDAL Soumit Sankar. (2013). The effect of confinement on proteins inside silica tubes investigated using SANS. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-784

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Metadata

Experiment Parameters

  • Environment temperature

    25-85
  • Experiment energy

    4.5-40 A
  • Experiment moment

    0.009-2 A-1
  • Experiment res energy

    as available
  • Experiment res moment

    as available

Sample Parameters

  • Formula

    • Hemoglobin
    • SiO_2
  • Consistence

    powder
  • Space

    P 4_1 2_1 2
  • Unit cell A

    53.70
  • Unit cell B

    53.70
  • Unit cell C

    193.80
  • Alpha

    90.00
  • Beta

    90.00
  • Gamma

    90.00