SANS Study on Synchronization Kinetics on Clock Protein
A circadian clock in cyanobacteria is the simplest and interesting living oscillator. This oscillator consists of only three proteins, KaiA, KaiB and KaiC, and works in vitro with ATP. The oscillation process has been revealed by SAXS studies and so on. However, the synchronization mechanism in a large number of protein oscillators is still unknown. Recently, it is supposed that subunit exchange between KaiC proteins could be a possible candidate for the interaction making synchronization. Therefore, in this study, we will prove the existence of the subunit exchange between KaiC proteins and also reveal the relation between the subunit exchange and synchronization mechanism by time-resolve SANS experiment utilizing deuterated subunits: this technique has been established for measurement of subunit exchange by our group in the previous work.
Please note that you will need to login with your ILL credentials to download the data.
Download DataThe recommended format for citing this dataset in a research publication is in the following format:
Masaaki Sugiyama; HIRAI Mitsuhiro; KATO Koichi; MARTEL Anne; YAGI Hirokazu and ZACCAI Joseph. (2013). SANS Study on Synchronization Kinetics on Clock Protein. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-801
ILL has partnered with DataCite as the registration agency
for data persistent identifiers.