Structure of a biological trans-membrane electron conduit.
Many bacteria are able to respire using solid minerals and metals as electron acceptors in the nabsence of oxygen or other diffusible substrates. In Shewanella oneidensis this respiration requires a porin-cytochrome complex that assembles in the outer membrane. This complex is composed of a trans-membrane porin MtrB, a periplasmic deca-haem cytochrome MtrA and a surface exposed outer membrane cytochrome MtrC. We intend to obtain information on the shape and size of the MtrCAB complex, which will inform on the orientation of exposed MtrC as well as whether MtrA either fully or partially enters MtrB, thus providing evidence for MtrB functioning as a sheath for MtrA. We have already collected data on SANS2D at the UK ISIS facilty using MtrCAB in different concentrations of deutereated buffer and have prepared samples in deuterated detergents ready for analysis. By collecting a full set of scattering measurements we hope to be able to generate a molecular envelope of the full MtrCAB cytochrome.
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Thomas Andrew Clarke; Marcus J Edwards; MARTEL Anne; SCOTT David and Gaye White. (2015). Structure of a biological trans-membrane electron conduit.. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-831
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