Investigation of PA pore translocation through lipid nanodiscs using SANS and Neutron Reflectometry
Bacillus anthracis produces three virulence factors that lead to profound organismal toxicity. Toxicity is dependent upon the delivery to the cytosol of two enzymatic proteins. The mechanism employed requires a pore forming protein, Protective Antigen (PA). Understanding the mechanism of protein translocation through the PA pore has important implications as it provides opportunities to develop effective neutralising therapies against bioterrorism, as well as novel drug delivery technologies. The hypothesis we wish to test, using neutron reflectometry and small angle scattering, relates to the interaction of the individual components and the capacity for the PA pore to relax allowing for stable macromolecules to translocate accessing the cytosol of a cell. We propose to create a model system to investigate this using lipid nanoparticles.
Please note that you will need to login with your ILL credentials to download the data.
Download DataThe recommended format for citing this dataset in a research publication is in the following format:
Paul D. R. Dyer; BARKER Robert; CATTOZ Beatrice and SHORTER Susan. (2015). Investigation of PA pore translocation through lipid nanodiscs using SANS and Neutron Reflectometry. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-848
ILL has partnered with DataCite as the registration agency
for data persistent identifiers.